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Fraction Collectors: Accurate and Automated Collection of Purified Samples in Separation Processes

Fraction collectors are essential and central tools in laboratories involved in separation processes based on chromatography (such as HPLC, FPLC, GC) or filtration. They enable controlled, automated, and precise collection of fractions from the outflow stream of a separation column, according to predefined parameters. This instrument is critical for isolating specific components from complex mixtures for subsequent analysis, characterization, or further use.

Principles of Operation, Flexibility, and Control in the Collection Process

The operating principle of a fraction collector is based on automatically diverting the eluent exiting the separation column into different collection vessels in a sequential manner. The transition between collection vessels is performed according to criteria defined by the user, such as elapsed time, collected volume, or most commonly – peak detection using a detector connected in series with the chromatography system (for example, a UV/Vis detector). The ability to accurately collect the desired peak allows for efficient isolation of the compound of interest at a high degree of purity.

Modern fraction collectors offer great flexibility in terms of collection vessels (test tubes, vials, microplates), compatibility with various volumes and flow rates, and seamless integration with chromatography system control software. The automation provided by fraction collectors dramatically reduces the need for manual monitoring, saves valuable time, minimizes human error, and ensures high reproducibility and reliability of the collection process, which are critical in research and quality control. They provide precise, unattended collection, allowing researchers to focus on other tasks while maintaining the integrity of their valuable samples.

Key Applications of Fraction Collectors in Laboratories

The use of fraction collectors is widespread and diverse across various laboratories:

  • Liquid Chromatography (HPLC, FPLC): Accurate fraction collection of peptides, proteins, nucleic acids, small molecules, and various chemical or biological compounds after analytical or preparative separation.
  • Sample Purification: Isolation of specific components from complex matrices in the pharmaceutical, biotechnology, food, and chemical industries for purification purposes.
  • Gas Chromatography (GC): Collection of separated volatile compounds for subsequent analysis or characterization (less common than with liquid chromatography).
  • Automated Assays and Quality Control: Collecting fractions based on timed intervals or specific events for serial testing or monitoring processes over time.
  • Method Development: Assisting in the development and optimization of new separation methods by facilitating easy collection and analysis of fractions.

Whether you need to isolate trace amounts for analysis or collect larger quantities for material purification, an efficient and accurate fraction collector is critical for the success of your separation process.

Leading Fraction Collectors from Labotal: Reliability, Accuracy, and Professional Support

Labotal is proud to offer a selection of fraction collectors from the leading and most reputable brands in the field of chromatography. We bring systems to your lab that are characterized by uncompromising performance, maximum collection accuracy, long-term durability, and advanced control technologies. The fraction collectors we supply are designed to work seamlessly with various chromatography systems and offer a range of options for customization to your specific needs. While specific brands can vary, our portfolio typically includes systems from manufacturers renowned for their reliability in demanding lab environments.

Choosing a fraction collector from Labotal means acquiring a complete solution that includes not only top-tier equipment but also the knowledge and experience of our expert team. We provide professional consultation to help you select the appropriate model, ongoing technical support, and training for optimal system operation. We are committed to the success of your separation processes in the lab and providing the most reliable tools that will enable you to achieve the most accurate results.

We invite you to explore Labotal’s diverse catalog of fraction collectors and discover how our automated collection solutions can enhance the efficiency and accuracy of your laboratory’s separation processes. For detailed consultation or a customized quote tailored to your needs, please contact our specialists.

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